| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Lucy Beckham "A" | Elliott Murphy, Finley Bennett, Scarlett True, Kate Spellane | 1:56.28 | |
| 2 | Cane Bay "A" | Fiona Haefner, Mia Stephen, Madeline Degeorge, Josie Mullen | 1:56.78 | |
| 3 | Lucy Beckham "B" | Claire Heinemann, Madeline O'Shea, Alexis Arrants, Margaret Carroll | 2:05.84 | |
| 4 | Cane Bay "B" | Ava Noble, Eva DeGeorge, Ella Noble, Avelyn Froetschner | 2:08.53 | |
| 5 | Charleston Charter for Math and Science "A" | Lilyanna Cates, Maddie Fletcher, Christina Leshko, Kiersten Cannon | 2:26.79 |