| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | LEXINGTON "A" | Reagan Taylor, Angelina Luo, Riley Halloran, Lyla Jones | 2:19.97 | |
| 2 | LEXINGTON "B" | Aubriana Keller, Jaclyn Liebenrood, Iva Francis, Arlie Eubanks | 2:49.11 | |
| 3 | White Knoll "A" | Isabella Steele, Ariana Kinzie, Anabelle Rousseau, Isabel Blevins | 2:55.50 | |
| 4 | Gilbert "A" | Jordan Humble, Alexandra Whitt, Ryan Humble, Mollie Wilson | 3:15.50 | |
| 5 | Mid-Carolina "A" | Miriam Eshaak, Baylee Willetts, Tailee Followell, Olivia Haskett | 3:17.12 | |
| DQ | White Knoll "B" | Lilliana Tinajero, Hailee Gunter, Savannah Campbell, MacKenzie Goff | — |