| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Hartsville "A" | Sophia Rhodes, Sofia Elias, Anna Hill-Brito, Alice Carr | 2:21.96 | |
| 2 | Wilson "A" | Diya Bharadwaj, Morgan Krawiec, Eden Weinmann, Sierra Estridge, Puja Bharadwaj, Rani Gopal | 2:22.94 | |
| 3 | South Florence "A" | Daija Torian, Caroline McInville, Deava Duvall, Anna Maxwell | 2:29.64 | |
| DQ | Governor's School "A" | Tanishka Agarwal, Caroline Gallup, Jane Salter, Ellison Foster | — |