| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | West Ashley "A" | Isabella Luciano, Ashlyn Cook, Soleil Smith, Clara Woolum | 2:22.14 | |
| 2 | James Island Charter "A" | Kaitlyn Frear, Neve Dutrow, Sophia Evans, Kaleigh Johnson | 2:25.40 | |
| 3 | James Island Charter "B" | Anna Yonce, Lydia Nolan, Mary Joy, Leila Curlett | 2:34.12 | |
| 4 | West Ashley "C" | Madison Lay, Caroline Brown, Tallulah Branton, Emma Griggs | 2:38.59 | |
| 5 | James Island Charter "C" | Darby Sheridan, June Houser, Scarlett Kraft, Marin Larson | 3:00.13 | |
| DQ | West Ashley "B" | Jane Robbins, Gabriela Ross, Harper Hanna, Lillian Parks | — |