| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Academic Magnet "A" | Leah Whipkey, Judith Suttle, Whitney Gwisc, Isa Jickling | 2:02.47 | |
| 2 | Porter-Gaud "A" | Anne Hyde, Sloan Pittard, Harriet Kelley, Zuri Swain | 2:03.15 | |
| 3 | Ashley Hall "A" | Ella Sidebottom, Amelia DeWolf, Amelia Lennon, Margaret Sidebottom | 2:10.14 | |
| 4 | Porter-Gaud "B" | Molly Doherty, Caitlin Baden, Lizzie Meden, Lilah Kowalski | 2:18.33 | |
| 5 | Academic Magnet "C" | Finley Bowers, Ruth Russell, Gabriella Perrone, Tiffani Tatzel | 2:21.50 | |
| 6 | Ashley Hall "B" | Leila Toporek, Kate Lennon, Olivia Gaines, Mae Wurzelbacher | 2:30.96 | |
| 7 | Porter-Gaud "C" | Finola Donahue, Eliana Nassar, Hollie King, Ann McIntosh | 2:35.61 | |
| DQ | Academic Magnet "B" | Charlotte Connors, Priya Cardella, Julia Schady, Julia Koch | — |