| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Chapin "A" | Ella O'Shaughnessey, Sydney Barkevich, Jacqueline Bonavilla, Ashlyn Newman | 1:59.25 | |
| 2 | Chapin "B" | Isabel Brownfield, McKenzie Metts, Madeleine Edwards, Delaney Gallagher | 2:08.32 | |
| 3 | Dreher "A" | Mira Wachtel, Zara Shah-Fuente, Hazle Ligon, Annabelle Kip | 2:20.19 | |
| 4 | American Leadership "A" | Eleanor Beers, Maria Nava, Charlotte Bell, Mary Hardaway | 2:30.06 | |
| 5 | Chapin "C" | Kaylee Mixon, Brynna Koorsen, Bailey Ceron Hernandez, Olivia Volk | 2:43.00 | |
| 6 | Strom Thurmond "A" | Emalyn Cockrell, Sara Willis, Olivia Stevens, Lucinda Whatley | 2:53.91 | |
| 7 | Mid-Carolina "A" | Tailee Followell, Baylee Willetts, Miriam Eshaak, Olivia Haskett | 2:56.00 |