| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Daniel "A" | Mary Greene, Piper Finneran, Elizabeth Russell, Bailey Choi | 1:59.25 | |
| 2 | Walhalla "A" | Ava Schuller, Perryn Poore, Audrey Burkhart, Gabriella Haubert | 2:12.84 | |
| 3 | Travelers Rest "A" | Isabella Brock, Lillie Prescott, Jayna Laven, Annabelle Taylor | 2:18.34 | |
| 4 | Daniel "B" | Melanie McKinnon, Glory Marshall, Jocelyn Campbell, McKenna Craig | 2:24.75 | |
| 5 | Travelers Rest "B" | Kali Anderson, Aleena Dixon, Ava Garrett, Riley Stokes | 2:42.18 | |
| DQ | Seneca "A" | Zoee Palmer, Lana Crain, Abigail Eaker, Rebekah Collins | — | |
| DQ | Walhalla "B" | Olivia Sanchez, Ella Hill, Nora Vosburgh, Camila Torres | — |