| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Lucy Beckham "A" | Finley Bennett, Kate Spellane, Scarlett True, Elliott Murphy | 2:12.50 | |
| 2 | West Ashley "A" | Sofia Savic, Cora Flanigan, Soleil Smith, Lila Perlmutter | 2:15.69 | |
| 3 | Lucy Beckham "B" | Delaney Bruce, Madeline O'Shea, Alexis Arrants, Claire Heinemann | 2:16.41 | |
| 4 | Ashley Hall "A" | Amelia Lennon, Ella Sidebottom, Margaret Sidebottom, London Edwards | 2:23.78 | |
| 5 | West Ashley "B" | Jane Robbins, Gabriela Ross, Harper Hanna, Isabella Luciano | 2:34.06 | |
| 6 | West Ashley "C" | Caroline Brown, Julia Hodges, Tallulah Branton, Lillian Parks | 2:40.80 | |
| DQ | West Ashley "D" | Alexandra Doose, Emma Green, Emma Griggs, Kelsey Linker | — |