| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | River Bluff "A" | Anna Ray, Ellie Wells, Caroline Wells, Keelyn Davis | 2:11.26 | |
| 2 | Spring Valley "A" | Isabella Oliveira, Jasmine Emokpae, Farrah Ballouli, Camille Anderson | 2:21.16 | |
| 3 | River Bluff "B" | Lore Stabler, Maddison Honour, Sydney Beeler, Kendal Eubanks | 2:41.96 | |
| 4 | Spring Valley "B" | Tori Li, Evthokia Deligiannidis, Annabelle Marrin, Greher Sexton | 2:52.09 | |
| DQ | River Bluff "C" | Sloka Pulijala, Caroline Cully, Jasmine Silva Velasco, Elana Jahnke | — |