| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Porter-Gaud "A" | Anne Hyde, Sloan Pittard, Harriet Kelley, Zuri Swain | 2:04.60 | |
| 2 | Ashley Hall "A" | London Edwards, Kate Lennon, Amelia Lennon, Margaret Sidebottom | 2:11.19 | |
| 3 | Northwood Academy "A" | Bailee Ball, Brooke Schwartzberg, Bella Bornfleth, Lila Kate Arntz | 2:13.29 | |
| 4 | Northwood Academy "B" | Mia Bumba, EmmyLou Parker, Maggie Garver, Chloie Donaldson | 2:31.75 | |
| 5 | Ashley Hall "B" | Mae Wurzelbacher, Callan Jessup, Mia Neskey, Vivienne Albert | 2:42.18 |