| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Eastside "A" | Darcy O'Sullivan, Anna Barr, Millie Conner, Lauren York | 1:57.09 | |
| 2 | Eastside "B" | Ana Ballinger, Leila Kluttz, Harper Penny, Laila Harvey | 1:59.57 | |
| 3 | Byrnes "A" | Izabella Strawn, Addison Bowen, Savannah Luttrell, Reese Seman | 2:01.13 | |
| 4 | Eastside "C" | Marion Bolt, Hayden Taylor, Emma Camp, Adelaide Hankins | 2:04.56 | |
| 5 | Byrnes "B" | Nyah Henderson, Elizabeth Brooks, Bailey Williamson, Caroline McCarty | 2:15.43 | |
| 6 | Boiling Springs "A" | Claire Miller, Cerys Ewing, Alexa Boykin, Bevin Hardy | 2:17.59 | |
| 7 | Eastside "D" | Emma Jones, Mary Chilton, Victoria Maynard, Bridget Parker | 2:20.34 | |
| 8 | Byrnes "C" | Kaleigh West, Mackenzi Ogborn, Hope Slemenda, Hallie Herzberg | 2:36.13 |