| Place | Team | Swimmers | Time | Splits |
|---|---|---|---|---|
| 1 | Cane Bay "A" | Fiona Haefner, Ava Noble, Madeline Degeorge, Josie Mullen | 1:58.32 | |
| 2 | James Island Charter "A" | Anna Yonce, Neve Dutrow, Sophia Evans, Leila Curlett | 2:11.18 | |
| 3 | Cane Bay "B" | Avelyn Froetschner, Eva DeGeorge, Ella Noble, Jillian Hinkle | 2:16.25 | |
| 4 | James Island Charter "B" | Kaitlyn Frear, Marin Larson, Kaleigh Johnson, Elliotte Burch | 2:23.49 |